MACSQuant 16 Analyzer

Miltenyi Biotec MACSQuant 16 Amalyzer

The MACSQuant Analyzer 16 is a benchtop, automated flow cytometer engineered for multi-parameter cell analysis, offering convenience, sensitivity, and flexibility for both research applications and advanced cell therapy workflows.

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As part of the MACSQuant product line, this model improves upon previous generations by delivering 16 optical parameters (i.e., multiple fluorescence detection channels) while maintaining a compact footprint and a high degree of automation.

Typical applications include immunology, immunophenotyping, cell and gene therapy (CGT) analytics, quality control processes, and general cell biology research.

Key Features & Capabilities
Highlighted advantages and system features:

Feature Description / Details
Optics & Detection
- Outfitted with three spatially separated lasers: 405 nm, 488 nm, and 640 nm.
- Provides 16 detection/optical parameters (multiple fluorescence channels, plus scatter signals).
- Signal detection covers FSC (forward scatter), SSC (side scatter), and multiple fluorescence channels (e.g., V1–V5, B1–B6, R1–R3), each equipped with optimized photomultiplier tubes (PMTs).

Fluidics / Sample Handling
- Minimum sample uptake: as little as 1 µL (recommended ~25 µL for precise volumetric counting).
- Adjustable sample flow rates: 25, 50, or 100 µL/min, or automated regulation to achieve target event rates (e.g., 500, 1,000, or 2,000 events/second).
- Maximum event rate: up to approximately 15,000 events/second (ALT mode).
- Compatible with various sample vessels, including 1.5, 2, and 5 mL tubes, tube racks, and 96-well plates. Transition between tubes and plates is user-friendly.
- Utilizes an automated robotic needle arm for sample aspiration, thorough mixing, and automated washing of both the injection port and needle before and after runs.

Automation & Workflow
- Full automation for maintenance routines, PMT gain calibration, fluorescence compensation, sample acquisition, and analysis.
- "Express Modes" (pre-set protocols) and templates support reproducible sample acquisition and data processing workflows.
- Harmonization features for cross-instrument and multi-site data alignment, promoting data standardization for multi-center studies.
- Regulatory and compliance support: offers 21 CFR Part 11-compatible features (audit trail, user management, electronic signatures, and reporting), making it ideal for clinical and translational research labs.

Sensitivity & Performance
- High fluorescence sensitivity: e.g., for standard dyes — FITC < 110 MESF, PE < 75 MESF, APC < 100 MESF
- High precision:

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